The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. Random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. But RAPD is infamous for its non-reproducibility because of various factors. So, in this work, an attempt was made to study its reproducibility in three Oryza sativa (Rice) varieties. Three different primers were tested, of which two reproducible RAPD primers were chosen. To ascertain their reproducibility, PCR was repeated three times for molecular profiling of random Restorer lines and confirmed their reproducibility.
RAPD markers, rice cultivars, reproducibility.