Direct amplification of crime scene samples has become a trending method in recent years. Many laboratories worldwide longed for this type of method in order to reduce case backlogs, save cost for extraction, save time and human labour. MicroFLOQ® swabs offered direct amplification method with promising evaluation results. Not only in saving time and cost for eliminating extraction procedure, this study has found that with a minimum of 8μl of GlobalFiler™ Express Kit (GFE), a full DNA profile could be generated from both buccal swab samples and blood samples. At 25 cycles, buccal swab samples could generate full profiles with 8μl of GFE while blood swabs require 27 cycles with the same volume of GFE. These results were a stepping stone to achieve a no- extraction procedure for screening purposes of crime scene samples. Thus, selective sampling could be carry out to fit the purpose of reducing cases turnaround time and budgeting for DNA laboratories.
direct PCR, buccal sample, bloodstained swab, microFLOQ®, GlobalFiler™ Express