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ES Journal of Microbiology

DOI: 10.59152/ESJM/1014

ISSN: 2768-0584

Synthesis and Characterization of Silver Nanoparticles as Strong Antimicrobial Agents Using Bacteriocin of A Novel Probiotic Strain of Lactobacillus Pentosus S6 (KU92122)

  • Research Article

  • Nivedita Sharma*, Shakshi Sharma, Pooja Sharma and Moksharthi Sharma
  • Microbiology Research laboratory, Department of Basic Sciences, Dr. Y S Parmar University of Horticulture and Forestry Nauni- Solan, Himachal Pradesh, India
  • *Corresponding author: Nivedita Sharma, Microbiology Research laboratory, Department of Basic Sciences, Dr. Y S Parmar University of Horticulture and Forestry Nauni- Solan, Himachal Pradesh, India
  • Received: June 20, 2022; Accepted: July 05, 2022; Published: July 08, 2022

Abstract

The goal of this study was to use bacteriocin from a probiotic strain of Lactobacillus pentosus S6 (KU92122) to synthesize innovative strong silver nanoparticles (AgNPs). UV-Vis spectroscopy was used to analyze the synthesized nanoparticles, and the maximum absorbance was found to be approximately 450 nm. AgNPs produced were spherical in shape, with an average size of 50 nm, as validated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to confirm the presence of distinct functional groups on the surface of nanoparticles. The positive antibacterial activity of produced silver nanoparticles was tested against multidrug resistant bacteria such as Staphylococcus aureus and Bacillus cereus, as well as challenging plant pathogens such as Aspergillus niger, Pythium aphanidermatum, Fusarium oxysporum, and Phytopthora parasitica with an apparent aim to use these AgNPs with bacteriocins as effective antimicrobial agents that can be employed at broader scale in clinical and biocontrol applications especially in medicine, agriculture and the food processing sectors.

Keywords

Silver Nanoparticles; Scanning electron microscopy; Transmission electron microscopy; Fourier transform infrared spectroscopy; Antimicrobial potential