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ES Journal of Clinical Medicine

ISSN: 2768-010X

Loop-Mediated Isothermal Amplification and its Application in Rapid Detection of Microorganisms

  • Review Article

  • Han M, Wu X and Yu D*
  • Department of Clinical Laboratory, Zhejiang University School of Medicine, Hangzhou, China
  • *Corresponding author: Daojun Yu, Department of Clinical Laboratory, Affiliated Hangzhou First People’s Hospital, Zhejiang Chinese Medical University, Hangzhou, China
  • Received: Nov 05, 2020; Accepted: Nov 20, 2020; Published: Nov 23, 2020


Loop-Mediated Isothermal Amplification (LAMP) is a new nucleic acid amplification technique developed by Japanese scientist Notomi [1] in 2000, which is concerned by, scholars all over the world and relevant government departments. In just a few years, LAMP has been successfully applied to the detection of diseases caused by various viruses, bacteria and parasites, safety inspection of food and cosmetics and rapid diagnosis of import and export quarantine. The advantages of LAMP method are as follows: high sensitivity (2-5 orders of magnitude higher than traditional PCR method); short reaction time (30~60min can complete the reaction); no special instruments are required for clinical use; easy to operate. Disadvantages of LAMP method: high sensitivity, easy to form aerosol pollution once opened, coupled with the fact that most domestic laboratories cannot be strictly divided, the problem of false positive is more serious, the requirement of primer design is relatively high, and the genes of some diseases may not be suitable for LAMP method. This review mainly describes the principle, types, application fields of loop-mediated isothermal amplification, and the characteristics of Bst DNA polymerase, detection methods of amplification products andother related aspects.


Loop-Mediated Isothermal Amplification; Isothermal Amplification Technique; Bst DNA polymerase; Microorganisms; Detection