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ES Journal of Agriculture and Current Research

Assessment of Genetic Diversity of Dioscorea Praehensilis (Berth.) Collected from Central Region, Ghana Using Simple Sequencerepeat (SSR) Markers

  • Research Article

  • Adeyinka S. Adewumi1,2, Kingsley J. Taah1, Paul A. Asare1, Michael O. Adu1and Paterne Agre2*
  • 1Department of Crop Science, School of Agriculture, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana
  • 2Yam Improvement Program, International Institute of Tropical Agriculture, Ibadan, Nigeria
  • *Corresponding author: Paterne Agre, Yam Improvement Program, International Institute of Tropical Agriculture, Ibadan, Nigeria
  • Received: Apr 02, 2020; Accepted: Apr 26, 2020; Published: Apr 30, 2020

Abstract

In Ghana, Dioscorea praehensilis (Berth.) is one of the semi-wild yam cultivated speciesespecially in the cocoa grown regions. Recently, D.praehensilis has been reported to contribute to food security of the small households in the region. In this studywe evaluated the genetic diversityof 43 D. praehensiliscollected from three regions in Ghana using simple sequence repeat (SSR) markers. Using 11 SSR markers, a total of 99 number of alleles were generated with an average of 8.48 alleles per locus. The mean gene diversity was 0.81, mean polymorphism information content was 0.82 while mean Shannon information index was 1.94. Principal coordinate analysis (PCoA) revealed a contribution of 40.16% of the first three coordinate axes and grouped the 43 Genotypes into 2 groups while hierarchical cluster through UPGMA revealed the presence of 3 main clusters. Molecular variance (AMOVA) revealed low genetic diversity among the three genetic populations. Results from this study revealed the need to conduct extensive germplasm collection across the country where D. praehensilis are cultivated as source of food to really understand the genetic baseline for its proper utilization in the breeding program as source of novel genes.

Keywords

Genetic diversity, Dioscorea praehensilis, SSR markers, Ghana